rabbit polyclonal anti aurora a pt288 (Novus Biologicals)
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rabbit polyclonal anti aurora a pt288
Rabbit Polyclonal Anti Aurora A Pt288, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+aurora+a+pt288/Aurora+A+%5Bp+Thr288%5D+Antibody/pm29276128-484-38-43
Average 93 stars, based on 13 article reviews
Rabbit Polyclonal Anti Aurora A Pt288, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti+aurora+a+pt288/Aurora+A+%5Bp+Thr288%5D+Antibody/pm29276128-484-38-43
Average 93 stars, based on 13 article reviews
rabbit polyclonal anti aurora a pt288 - by Bioz Stars,
2026-09
93/100 stars
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Staining:Article Title: Tension-Induced Error Correction and Not Kinetochore Attachment Status Activates the SAC in an Aurora-B/C-Dependent Manner in Oocytes. Article Snippet: For whole-mount immunofluorescence, oocytes were first transferred in chambers coated with Concanavaline A (Sigma-Aldrich) in M2 PVP (Polyvinylpyrrolidone from Merck-Millipore) medium, followed by centrifugation at 1400 rpm for 13 min, all at 38 C. Prior to fixation, oocytes were incubated in a cold treatment solution (0.08MPIPES, 1mMMgCl2, pH 7.4) on top of an ice-water bath tomaintain a temperature of 4 C for 4 min. Oocytes were then fixed in a formaldehyde solution (BRB80 medium with 0.3% Triton X-100, 1.9% formaldehyde (Sigma-Aldrich)) at 38 C. For chromosome spreads, oocytes were fixed in a spread solution (1% paraformaldehyde, 0.15% Triton X-100 and 3mM 1,4 Dithiothreitol, all from Sigma-Aldrich) at room temperature [52]. .. Antibody staining was done using the following antibodies at the indicated dilutions: human CREST serum autoimmune antibody against centromere (Immunovision, HCT-0100, 1:100), mouse monoclonal anti-alpha-tubulin (DM1A) coupled to FITC (Sigma-Aldrich, F2168, 1:100), rat monoclonal anti-H3pS28 (Abcam, #ab10543, 1:500), |